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TitleSemi-preparative supercritical chromatography scale plant for polyunsaturated fatty acids purification
Publication TypeJournal Article
Year of Publication2013
AuthorsMontanes, F., Catchpole O.J., Tallon S., Mitchell K., and Lagutin K.
JournalJournal of Supercritical Fluids
Pagination46 - 54
Date Published2013
ISSN08968446 (ISSN)
Keywords10.1016/j.supflu.2012.11.018, algae, Algae oil, Carbon dioxide, Esterification, Esters, Fatty acid ethyl ester, fish oil, gas chromatography, particle size, Polyunsaturated fatty acids, purification, Semi-preparative, Super critical fluid chromatography
AbstractA one step supercritical fluid chromatography process has been developed using a semi-preparative supercritical fluid chromatography scale plant designed and built in-house, for isolation and purification of polyunsaturated fatty acid ethyl esters from different origins (fish and algae). A wide range of experimental conditions (pressure, temperature, sample load, co-solvent rate and CO2 flow rate); columns (stationary phase and particle size); and UV/vis detectors have been investigated to optimise the isolation and purification of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) from an algae oil rich in EPA, and a fish oil rich in EPA and DHA. The particle size, followed by the stationary phase, sample loading, temperature/pressure and feed oil composition, were found to be the most important parameters for achieving separation. Under optimised conditions, EPA at greater than 95% purity from both oils and DHA at greater than 80% purity from fish oil was obtained in a single pass and without co-solvent. © 2012 Elsevier B.V. All rights reserved.

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