| Title | Semi-preparative supercritical chromatography scale plant for polyunsaturated fatty acids purification |
| Publication Type | Journal Article |
| Year of Publication | 2013 |
| Authors | Montanes, F., Catchpole O.J., Tallon S., Mitchell K., and Lagutin K. |
| Journal | Journal of Supercritical Fluids |
| Volume | 79 |
| Pagination | 46 - 54 |
| Date Published | 2013 |
| ISSN | 08968446 (ISSN) |
| Keywords | 10.1016/j.supflu.2012.11.018, algae, Algae oil, Carbon dioxide, Esterification, Esters, Fatty acid ethyl ester, fish oil, gas chromatography, particle size, Polyunsaturated fatty acids, purification, Semi-preparative, Super critical fluid chromatography |
| Abstract | A one step supercritical fluid chromatography process has been developed using a semi-preparative supercritical fluid chromatography scale plant designed and built in-house, for isolation and purification of polyunsaturated fatty acid ethyl esters from different origins (fish and algae). A wide range of experimental conditions (pressure, temperature, sample load, co-solvent rate and CO2 flow rate); columns (stationary phase and particle size); and UV/vis detectors have been investigated to optimise the isolation and purification of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) from an algae oil rich in EPA, and a fish oil rich in EPA and DHA. The particle size, followed by the stationary phase, sample loading, temperature/pressure and feed oil composition, were found to be the most important parameters for achieving separation. Under optimised conditions, EPA at greater than 95% purity from both oils and DHA at greater than 80% purity from fish oil was obtained in a single pass and without co-solvent. © 2012 Elsevier B.V. All rights reserved. |
| URL | http://www.scopus.com/inward/record.url?eid=2-s2.0-84883657784&partnerID=40&md5=ad4d8d7c863c6a7209ca63b062fde0c3 |
| DOI | 10.1016/j.supflu.2012.11.018 |
