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TitleQuantitative Analysis of Dairy Phospholipids by 31P NMR
Publication TypeJournal Article
Year of Publication2009
AuthorsMacKenzie, A., Vyssotski M., and Nekrasov E.
JournalJAOCS, Journal of the American Oil Chemists' Society
Pagination757 - 763
Date Published2009
ISSN0003021X (ISSN)
Keywords<sup>31</sup> P NMR, Chromatographic analysis, Dairy products, Extraction, Lipid extraction, NMR analysis, nuclear magnetic resonance, nuclear magnetic resonance spectroscopy, Phosphatidylcholine, phosphatidylethanolamine, Phosphatidylinositol, Phosphatidylserine, Phospholipids, Protein concentrates, quantitative analysis, Quantitative measurement, Routine analysis, Sample analysis, Sodium, Sodium cholate, Sphingomyelin, thin layer chromatography, TLC, TLC systems
Abstract31P NMR analysis of samples prepared in a sodium cholate detergent system was assessed as a method for the quantitative measurement of dairy phospholipids. Major phospholipid (PL) classes measured included: phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylinositol (PI), sphingomyelin (SM) and dihydrosphingomyelin (DHSM). The 31P NMR method was validated by comparison with a quantitative two-dimensional thin-layer chromatography (2D-TLC) technique. The 2D-TLC system was more sensitive, able to detect some minor compounds not observed by 31P NMR. However, 31P NMR is more suited to routine analysis, with sample analysis taking 36 min. The method was also more versatile and sample analysis was possible on high phospholipid containing materials without prior lipid extraction (e.g. buttermilk protein concentrate, beta serum liquid). © AOCS 2009.

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