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TitlePyrinomonas methylaliphatogenes gen. nov., sp. nov., a novel group 4 thermophilic member of the phylum Acidobacteria from geothermal soils
Publication TypeJournal Article
Year of Publication2014
AuthorsCrowe, M.A., Power J.F., Morgan X.C., Dunfield P.F., Lagutin K., Rijpstra W.I.C., Rijpstra I.C., Damste J.S. Sinninghe, Houghton K.M., Ryan J.L.J., and Stott M.B.
JournalInternational Journal of Systematic and Evolutionary Microbiology
IssuePART 1
Pagination220 - 227
Date Published2014
ISSN14665026 (ISSN)
KeywordsAcidobacteria, aerobic metabolism, alginic acid, Ammonia, arabinose, article, bacterial strain, Bacterial Typing Techniques, bacterium, Base Composition, casamino acid, catalase, cell growth, Chloracidobacterium thermophilum, dicarboxylic acid, DNA, Bacterial, ether lipid, Extraction, Fatty Acids, formic acid, gene sequence, genomic DNA, glucose, glycerol ether, Gram negative bacterium, Hot Temperature, lactic acid, lipid, mannose, Molecular Sequence Data, new zealand, nitrate, nitrogen, nonhuman, nucleotide sequence, nutrient, oxidoreductase, peptone, pH, phenotype, phylogeny, phylum, priority journal, Pyrinomonas methylaliphatogenes, quinone derivative, RNA, Ribosomal, 16S, Sequence Analysis, DNA, soil analysis, Soil Microbiology, sucrose, Temperature, thermophilic bacterium, Urea, Vitamin K 2, xanthan, xylan, xylose, Yeast
AbstractAn aerobic, thermophilic, moderately acidophilic non-spore-forming bacterium, strain K22T, was isolated from geothermally heated soil at Mount Ngauruhoe, New Zealand. On the basis of 16S rRNA gene sequence similarity, K22T was shown to belong to subdivision 4 of the phylum Acidobacteria and to be most closely related to 'Candidatus Chloracidobacterium thermophilum' (86 %) and Blastocatella fastidiosa (86 %). Cells stained Gram-negative and were catalase and oxidase-positive. The major fatty acids detected were iso-C15: 0, iso-C17: 0, iso-C19: 0 and iso-C21: 0 when standard lipid extraction protocols were employed. Analysis of the total cell lipid acid hydrolysate also detected membrane-spanning and ether lipids, which made up approximately 40 % of the total membrane composition. These lipids included dicarboxylic (iso-diabolic) acid and the glyceryl ether of alkyl analogues of iso-C15: 0 and iso-diabolic acid. The G+C content of the genomic DNA was 59.6 mol% and the primary respiratory quinone was MK-8. Strain K22T grew at 50-69 °C with an optimum temperature of 65 °C and at pH 4.1-7.8 with an optimum growth pH of 6.5. NaCl tolerance was up to 1 % (w/v). Cells displayed a chemoheterotrophic and obligately aerobic metabolism. Cells grew on nutrient broth, alginate, arabinose, Casamino acids, glucose, lactate, formate, mannose, sodium alginate, peptone, sucrose, tryptone, xanthan, xylan, xylose and yeast extract. Nitrogen sources included nitrate, ammonium, urea, yeast extract and Casamino acids, but not dinitrogen gas. The distinct phylogenetic position and the phenotypic characteristics separate strain K22T from all other members of the class Acidobacteria and indicate that it represents a novel species and genus, for which the name Pyrinomonas methylaliphatogenes gen. nov., sp. nov. is proposed. The type strain of the type species is K22T (= DSM 25857T = ICMP 18710T). © 2014 IUMS.

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