Callaghan Innovation Research Papers

Back to Research Papers

TitlePaenibacillus darwinianus sp. nov., isolated from gamma-irradiated Antarctic soil
Publication TypeJournal Article
Year of Publication2014
AuthorsDsouza, M., Taylor M.W., Ryan J., MacKenzie A., Lagutin K., Anderson R.F., Turner S.J., and Aislabie J.
JournalInternational Journal of Systematic and Evolutionary Microbiology
IssuePART 4
Pagination1406 - 1411
Date Published2014
ISSN14665026 (ISSN)
KeywordsAntarctic Regions, Antarctica, article, bacterial growth, bacterial strain, Bacterial Typing Techniques, bacterium isolate, Base Composition, carbon utilization, cardiolipin, fatty acid analysis, Fatty Acids, flagellum, gamma irradiation, gamma radiation, gene sequence, genomic DNA, Hydrolysis, isoprenoid, maximum likelihood method, membrane fluidity, Molecular Sequence Data, nonhuman, nucleotide sequence, Paenibacillus, Paenibacillus daejeonensis, Paenibacillus darwinianus, Paenibacillus pinihumi, Paenibacillus tarimensis, phosphatidylethanolamine, phosphatidylglycerol, phospholipid, Phospholipids, phylogeny, priority journal, RNA 16S, RNA, Ribosomal, 16S, Sequence Analysis, DNA, Soil, Soil Microbiology, type strain, Vitamin K 2
AbstractA novel bacterium, strain BrT, was isolated from gamma-irradiated soils of the Britannia drift, Lake Wellman Region, Antarctica. This isolate was rod-shaped, endospore forming, Gram-stain-variable, catalase-positive, oxidase-negative and strictly aerobic. Cells possessed a monotrichous flagellum. Optimal growth was observed at 18 °C, pH 7.0 in PYGV or R2A broth. The major cellular fatty acid was anteiso-C15: 0 (63.4 %). Primary identified lipids included phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. Total phospholipid was 60 % (w/w) of the total lipid extract. MK-7 was the dominant isoprenoid quinone. The genomic DNA G+C content was 55.6 mol%. Based on 16S rRNA gene sequence similarity, strain BrT clusters within the genus Paenibacillus with similarity values ranging from 93.9 to 95.1 %. Phylogenetic analyses by maximum-likelihood, maximum-parsimony and neighbour-joining methods revealed that strain BrT clusters with Paenibacillus daejeonensis (AF290916), Paenibacillus tarimensis (EF125184) and Paenibacillus pinihumi (GQ423057), albeit with weak bootstrap support. On the basis of phenotypic, chemotaxonomic and phylogenetic characteristics, we propose that strain BrT represents a novel species, Paenibacillus darwinianus sp. nov. The type strain is BrT (= DSM 27245T = ICMP 19912T). © 2014 IUMS.

Back to top