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TitleOctanoate in human albumin preparations is detrimental to mesenchymal stromal cell culture
Publication TypeJournal Article
Year of Publication2015
AuthorsWong, W.-W., Mackenzie A.D., Nelson V.J., Faed J.M., and Turner P.R.
JournalStem Cells International
Date Published2015
ISSN16879678 (ISSN)
Keywords5' nucleotidase, activated leukocyte cell adhesion molecule, adenosine triphosphatase (potassium sodium), adipocyte, adult, article, cell differentiation, cell function, cell growth, cell isolation, cell population, colony forming cell, controlled study, endoglin, ex vivo study, fatty acid, Female, growth rate, Hermes antigen, human, human cell, human experiment, immunophenotyping, ion transport, mesenchymal stem cell, molecular biology, normal human, octanoic acid, osteoblast, pasteurization, priority journal, protein expression, serum albumin, stem cell culture, Thy 1 antigen, young adult
AbstractCell therapies hold great promise as the next major advance in medical treatment. To enable safe, effective ex vivo culture whilst maintaining cell phenotype, growth media constituents must be carefully controlled. We have used a chemically defined mesenchymal stromal cell culture medium to investigate the influence of different preparations of human serum albumin. We examined two aspects of cell culture, growth rate as measured by population doubling time and colony forming ability which is a representative measure of the stemness of the cell population. Albumin preparations showed comparative differences in both of these criteria. Analysis of the albumin bound fatty acids also showed differences depending on the manufacturing procedure used. We demonstrated that octanoate, an additive used to stabilize albumin during pasteurization, slows growth and lowers colony forming ability during ex vivo culture. Further to this we also found the level of Na+/K+ ATPase, a membrane bound cation pump inhibited by octanoate, is increased in cells exposed to this compound. We conclude that the inclusion of human serum albumin in ex vivo growth media requires careful consideration of not only the source of albumin, but also the associated molecular cargo, for optimal cell growth and behavior. © 2015 Way-Wua Wong et al.

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