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TitleA novel fatty acid, 12,17-dimethyloctadecanoic acid, from the extremophile Thermogemmatispora sp. (strain T81)
Publication TypeJournal Article
Year of Publication2012
AuthorsVyssotski, M., Ryan J., Lagutin K., Wong H., Morgan X., and Stott M.
Pagination601 - 606
Date Published2012
ISSN00244201 (ISSN)
Keywords12,17 dimethyloctadecanoic acid, article, Bacteria (microorganisms), bacterial growth, bacterial strain, bacterium identification, carbon nuclear magnetic resonance, Chloroflexi, Chloroflexi (class), fatty acid analysis, fatty acid synthesis, Fatty Acids, Gas Chromatography-Mass Spectrometry, Gram positive bacterium, lipid composition, Magnetic Resonance Spectroscopy, Mass Fragmentography, microbial activity, Molecular Structure, nonhuman, proton nuclear magnetic resonance, species difference, stearic acid, structure analysis, Thermogemmatispora, unclassified drug
AbstractThe major fatty acids of a novel species of Thermogemmatispora sp. (strain T81) from the phylum Chloroflexi were identified as i18:0 (42.8 % of total fatty acids), i19:0 (9.7 %), and i17:0 (5.9 %). Also observed was a number of unidentified fatty acids, including a major acid (16.3 %) with ECL of 19.04(BP1), and 18.76 (TGWAXMS A). GCMS revealed that this compound is a saturated 20-carbon atom fatty acid. 1H- and 13C-NMR, with 1H-1H-COSY and 1H-13C-HSQC experiments suggested the structure of dimethyl octadecanoic acid with isobranching, and an extra middle-chain methyl group. A pyrrolidide derivative demonstrated the characteristic gaps in GCMS indicating methyl branching at C12 and C17, which was eventually confirmed by a 1H-13C-HSQC- TOCSY experiment. This 12,17-dimethyloctadecanoic acid has not been previously detected or described in these organisms. However, a recent description of a phylogenetically related species of Thermogemmatispora (Yabe et al., Int J Syst Evol Microbiol 61:903-910, 2010), noted an unidentified 20:0 fatty acid with matching GC behavior and GCMS data to that of strain T81. These data suggest that Thermogemmatispora share an ability to synthesize the same fatty acid. A number of other dimethyl-branched fatty acids, namely 8,14-diMe 15:0; 12,15-diMe 16:0; 10,15-diMe 16:0; 12,16-diMe 17:0; 10,16-diMe 17:0; 12,17-diMe 18:0; 12,18-diMe 19:0; 14,19-diMe 20:0, were also identified in strain T81. © AOCS 2011.

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