| Title | A novel fatty acid, 12,17-dimethyloctadecanoic acid, from the extremophile Thermogemmatispora sp. (strain T81) |
| Publication Type | Journal Article |
| Year of Publication | 2012 |
| Authors | Vyssotski, M., Ryan J., Lagutin K., Wong H., Morgan X., and Stott M. |
| Journal | Lipids |
| Volume | 47 |
| Issue | 6 |
| Pagination | 601 - 606 |
| Date Published | 2012 |
| ISSN | 00244201 (ISSN) |
| Keywords | 12,17 dimethyloctadecanoic acid, article, Bacteria (microorganisms), bacterial growth, bacterial strain, bacterium identification, carbon nuclear magnetic resonance, Chloroflexi, Chloroflexi (class), fatty acid analysis, fatty acid synthesis, Fatty Acids, Gas Chromatography-Mass Spectrometry, Gram positive bacterium, lipid composition, Magnetic Resonance Spectroscopy, Mass Fragmentography, microbial activity, Molecular Structure, nonhuman, proton nuclear magnetic resonance, species difference, stearic acid, structure analysis, Thermogemmatispora, unclassified drug |
| Abstract | The major fatty acids of a novel species of Thermogemmatispora sp. (strain T81) from the phylum Chloroflexi were identified as i18:0 (42.8 % of total fatty acids), i19:0 (9.7 %), and i17:0 (5.9 %). Also observed was a number of unidentified fatty acids, including a major acid (16.3 %) with ECL of 19.04(BP1), and 18.76 (TGWAXMS A). GCMS revealed that this compound is a saturated 20-carbon atom fatty acid. 1H- and 13C-NMR, with 1H-1H-COSY and 1H-13C-HSQC experiments suggested the structure of dimethyl octadecanoic acid with isobranching, and an extra middle-chain methyl group. A pyrrolidide derivative demonstrated the characteristic gaps in GCMS indicating methyl branching at C12 and C17, which was eventually confirmed by a 1H-13C-HSQC- TOCSY experiment. This 12,17-dimethyloctadecanoic acid has not been previously detected or described in these organisms. However, a recent description of a phylogenetically related species of Thermogemmatispora (Yabe et al., Int J Syst Evol Microbiol 61:903-910, 2010), noted an unidentified 20:0 fatty acid with matching GC behavior and GCMS data to that of strain T81. These data suggest that Thermogemmatispora share an ability to synthesize the same fatty acid. A number of other dimethyl-branched fatty acids, namely 8,14-diMe 15:0; 12,15-diMe 16:0; 10,15-diMe 16:0; 12,16-diMe 17:0; 10,16-diMe 17:0; 12,17-diMe 18:0; 12,18-diMe 19:0; 14,19-diMe 20:0, were also identified in strain T81. © AOCS 2011. |
| URL | http://www.scopus.com/inward/record.url?eid=2-s2.0-84863429736&partnerID=40&md5=87d7592facbfdc9d4f4ff800286a4e68 |
| DOI | 10.1007/s11745-012-3668-z |
