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TitleMarinobacter salarius sp. nov. and marinobacter similis sp. nov., isolated from sea water
Publication TypeJournal Article
Year of Publication2014
AuthorsNg, H.J., M. Rez Ló Pez- Pé, Webb H.K., Gomez D., Sawabe T., Ryan J., Vyssotski M., Bizet C., Malherbe F., Mikhailov V.V., Crawford R.J., and Ivanova E.P.
JournalPLoS ONE
Date Published2014
ISSN19326203 (ISSN)
Keywordsarticle, bacterial DNA, bacterial gene, bacterial growth, bacterial strain, bacterium isolate, chemotaxonomy, Classification, controlled study, DNA base composition, DNA, Bacterial, gene sequence, genetics, gyrB gene, isolation and purification, Marine Biology, Marinobacter, Marinobacter adhaerens, Marinobacter algicola, Marinobacter flavimaris, Marinobacter hydrocarbonoclasticus, Marinobacter salarius, Marinobacter sediminum, Marinobacter similis, Mass spectrometry, microbiology, nonhuman, Nucleic Acid Hybridization, nucleotide sequence, phylogeny, RNA 16S, rpoD gene, sea water, Seawater, sequence homology, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Abstracte106514 Two non-pigmented, motile, Gram-negative marine bacteria designated R9SW1T and A3d10T were isolated from sea water samples collected from Chazhma Bay, Gulf of Peter the Great, Sea of Japan, Pacific Ocean, Russia and St. Kilda Beach, Port Phillip Bay, the Tasman Sea, Pacific Ocean, respectively. Both organisms were found to grow between 4uC and 40uC, between pH 6 to 9, and are moderately halophilic, tolerating up to 20% (w/v) NaCl. Both strains were found to be able to degrade Tween 40 and 80, but only strain R9SW1T was found to be able to degrade starch. The major fatty acids were characteristic for the genus Marinobacter including C16:0, C16:1v7c, C18:1v9cand C18:1v7c. The G+C content of the DNA for strains R9SW1T and A3d10T were determined to be 57.1 mol% and 57.6 mol%, respectively. The two new strains share 97.6% of their 16S rRNA gene sequences, with 82.3% similarity in the average nucleotide identity (ANI), 19.8% similarity in the in silico genome-to-genome distance (GGD), 68.1% similarity in the average amino acid identity (AAI) of all conserved proteincoding genes, and 31 of the Karlin's genomic signature dissimilarity. A phylogenetic analysis showed that R9SW1T clusters with M. algicola DG893T sharing 99.40%, and A3d10T clusters with M. sediminum R65T sharing 99.53% of 16S rRNA gene sequence similarities. The results of the genomic and polyphasic taxonomic study, including genomic, genetic, phenotypic, chemotaxonomic and phylogenetic analyses based on the 16S rRNA, gyrB and rpoD gene sequence similarities, the analysis of the protein profiles generated using MALDI-TOF mass spectrometry, and DNA-DNA relatedness data, indicated that strains R9SW1T and A3d10T represent two novel species of the genus Marinobacter. The names Marinobacter salarius sp. nov., with the type strain R9SW1T ( = LMG 27497T = JCM 19399T = CIP 110588T = KMM 7502T) and Marinobacter similis sp. nov., with the type strain A3d10T ( = JCM 19398T= CIP 110589T = KMM 7501T), are proposed. © 2014 Ng et al.

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