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TitleIsolation and structural characterisation of the major glycolipids from Lactobacillus plantarum
Publication TypeJournal Article
Year of Publication2012
AuthorsSauvageau, J., Ryan J., Lagutin K., Sims I.M., Stocker B.L., and Timmer M.S.M.
JournalCarbohydrate Research
Pagination151 - 156
Date Published2012
ISSN00086215 (ISSN)
Keywordsarticle, bacterial cell wall, carbohydrate analysis, Carbohydrate Conformation, Carbohydrate Sequence, carbon nuclear magnetic resonance, Chromatography, Gel, electrospray mass spectrometry, Fatty Acids, galactose, glucose, Glycerides, glycolipid, Glycolipids, heteronuclear multiple bond correlation, heteronuclear single quantum coherence, Lactobacillus, Lactobacillus plantarum, Lipids, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Molecular Structure, Natural products, Organic compounds, priority journal, proton nuclear magnetic resonance, Spectrometry, Mass, Electrospray Ionization, Stereoisomerism, Structural elucidation, Synthesis (chemical), tandem mass spectrometry
AbstractTo date, the structures of the glycolipids from Lactobacillus plantarum, a commonly used beneficial probiotic, have not been conclusively assigned. Herein, we report for the first time, the full characterisation of the four principal glycolipids of the L. plantarum cell wall using sugar, linkage and FAME analysis, as well as ESI-MS/MS and 1D- and 2D-NMR spectroscopy, and assign the major glycolipids as being: α-d-Glcp-diglyceride, α-d-Galp- (1→2)-α-d-Glcp-diglyceride, β-d-Glcp-(1→6)-α-d-Galp- (1→2)-6-O-acyl-α-d-Glcp-diglyceride and β-d-Glcp-(1→6)- α-d-Galp-(1→2)-α-d-Glcp-diglyceride. © 2012 Published by Elsevier Ltd.

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