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TitleInvitro fermentation of prebiotic oligosaccharides by Bifidobacterium lactis HN019 and Lactobacillus spp.
Publication TypeJournal Article
Year of Publication2014
AuthorsSims, I.M., Ryan J.L.J., and Kim S.H.
JournalAnaerobe
Volume25
Pagination11 - 17
Date Published2014
ISSN10759964 (ISSN)
Keywordsanion exchange chromatography, article, bacterial growth, bacterial strain, beta glucan, Bifidobacterium, Bifidobacterium animalis, Bifidobacterium animalis subsp. lactis, carbohydrate analysis, carbohydrate intake, Carbohydrate Metabolism, cell free system, Chromatographic analysis, chromatography, controlled study, disaccharide, fermentation, fructose oligosaccharide, galactose oligosaccharide, growth, development and aging, high performance liquid chromatography, in vitro study, inulin, Lactobacillus, Lactobacillus acidophilus, Lactobacillus rhamnosus, metabolism, microbial activity, monosaccharide, nonhuman, oligosaccharide, Oligosaccharides, prebiotic agent, Prebiotics, priority journal, probiotic agent, Probiotics, synbiotic agent, trisaccharide
AbstractThe utilisation of various prebiotic oligosaccharides by probiotic strains of Bifidobacterium lactis, Lactobacillus rhamnosus and Lactobacillus acidophilus was investigated in order to determine the synbiotic potential of various prebiotic/probiotic combinations. Analysis by HPLC and high-performance anion-exchange chromatography of the cell-free medium taken during growth of the three probiotic bacteria showed differences in the consumption of the various oligosaccharides. Analysis of galactooligosaccharides showed that both L.rhamnosus and B.lactis consumed mostly mono- and di-saccharide, while L.acidophilus consumed oligosaccharides up to trisaccharide. Both B.lactis and L.acidophilus utilised fructooligosaccharides and inulin, but showed different patterns of oligosaccharide consumption. Only L.rhamnosus grew on β-glucan oligosaccharides and preferentially consumed the trisaccharide. The results indicate the synbiotic potential of the various probiotic/prebiotic combinations, particularly L.acidophilus/galactooligosaccharides, L.acidophilus/fructooligosaccharides or inulin and L.rhamnosus/β-glucan oligosaccharides. © 2013 Elsevier Ltd.
URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84888337311&partnerID=40&md5=dc5e6839fa7024a4a30fae755a401c61
DOI10.1016/j.anaerobe.2013.11.001

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