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TitleThe identification and quantification of phospholipids from Thermus and Meiothermus bacteria
Publication TypeJournal Article
Year of Publication2014
AuthorsLagutin, K., MacKenzie A., Houghton K.M., Stott M.B., and Vyssotski M.
Pagination1133 - 1141
Date Published2014
ISSN00244201 (ISSN)
Keywords2' o (1,2 diacyl sn glycero 3 phospho) 3' o (alpha acetylglucosaminyl) n glyceroyl alkylamine, 2' o (1,2 diacyl sn glycero 3 phospho) 3' o (alpha galactosaminyl) n glyceroyl alkylamine, 2' o (1,2 diacyl sn glycero 3 phospho) 3' o (alpha glucosaminyl) n glyceroyl alkylamine, 2' o (1,2 diacyl sn glycero 3 phospho) n glyceroyl alkylamine(phosphatidylglyceroylalkylamine), 2' o (2 acylalkyldiol 1 o phospho) 3' o (alpha acetylglucosaminyl) n glyceroyl alkylamine, 2' o (2 acylalkyldiol 1 o phospho) n glyceroyl alkylamine, aliphatic amine, article, bacterial strain, carbohydrate analysis, Deinococcus-Thermus, electrospray mass spectrometry, galactosamine, glucosamine, lipid analysis, Meiothermus, Meiothermus ruber, nonhuman, phospholipid, phosphorus nuclear magnetic resonance, Thermus, Thermus filiformis, thin layer chromatography, unclassified drug
AbstractStructural identities of the major phospholipid (PL-2), minor phospholipid (PL-1) and trace phospholipid (PL-0) from representative strains of the genera Thermus and Meiothermus were established. Phospholipids were quantified using phosphorus-31 nuclear magnetic resonance (31P-NMR). The structures of the major phospholipid (PL-2) from Thermus filiformis MOK14.7 and Meiothermus ruber WRG6.9 were identified as 2′-O-(1,2-diacyl-sn-glycero-3-phospho)-3′-O-(α-N-acetylglucosaminyl)-N-glyceroyl alkylamine (GlcNAc-PGAA) and 2′-O-(2-acylalkyldiol-1-O-phospho)-3′-O-(α-N-acetylglucosaminyl)-N-glyceroyl alkylamine (GlcNAc-diolPGAA). Interestingly, M. ruber contained only a diacyl form of GlcNAc-PGAA (87 %), while T. filiformis contained both GlcNAc-PGAA (59 %) and GlcNAc-diolPGAA (18 %). The structures of the minor phospholipid (PL-1) were established as 2′-O-(1,2-diacyl-sn-glycero-3-phospho)-3′-O-(α-glucosaminyl)-N-glyceroyl alkylamine (GlcN-PGAA, 13 %) in T. filiformis and 2′-O-(1,2-diacyl-sn-glycero-3-phospho)-3′-O-(α-galactosaminyl)-N-glyceroyl alkylamine (GalN-PGAA, 19 %) in M. ruber. This is the first reliable discovery of phosphatidylglyceroylalkylamines modified by glucosamine or galactosamine with a free amino group. No signs of diol-based phosphatidylglyceroylalkylamines were found in PL-1 phospholipids. Similar to PL-2, trace phospholipid (PL-0) from T. filiformis contained both unsubstituted diol-based phosphatidylglyceroylalkylamine (diolPGAA) and PGAA, while M. ruber contained only free PGAA. Unlike analysis using TLC, the diol form of phosphatidylglyceroylalkylamines is clearly resolved from the diacyl form via 31P-NMR. © 2014 AOCS.

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