| Title | The identification and quantification of phospholipids from Thermus and Meiothermus bacteria |
| Publication Type | Journal Article |
| Year of Publication | 2014 |
| Authors | Lagutin, K., MacKenzie A., Houghton K.M., Stott M.B., and Vyssotski M. |
| Journal | Lipids |
| Volume | 49 |
| Issue | 11 |
| Pagination | 1133 - 1141 |
| Date Published | 2014 |
| ISSN | 00244201 (ISSN) |
| Keywords | 2' o (1,2 diacyl sn glycero 3 phospho) 3' o (alpha acetylglucosaminyl) n glyceroyl alkylamine, 2' o (1,2 diacyl sn glycero 3 phospho) 3' o (alpha galactosaminyl) n glyceroyl alkylamine, 2' o (1,2 diacyl sn glycero 3 phospho) 3' o (alpha glucosaminyl) n glyceroyl alkylamine, 2' o (1,2 diacyl sn glycero 3 phospho) n glyceroyl alkylamine(phosphatidylglyceroylalkylamine), 2' o (2 acylalkyldiol 1 o phospho) 3' o (alpha acetylglucosaminyl) n glyceroyl alkylamine, 2' o (2 acylalkyldiol 1 o phospho) n glyceroyl alkylamine, aliphatic amine, article, bacterial strain, carbohydrate analysis, Deinococcus-Thermus, electrospray mass spectrometry, galactosamine, glucosamine, lipid analysis, Meiothermus, Meiothermus ruber, nonhuman, phospholipid, phosphorus nuclear magnetic resonance, Thermus, Thermus filiformis, thin layer chromatography, unclassified drug |
| Abstract | Structural identities of the major phospholipid (PL-2), minor phospholipid (PL-1) and trace phospholipid (PL-0) from representative strains of the genera Thermus and Meiothermus were established. Phospholipids were quantified using phosphorus-31 nuclear magnetic resonance (31P-NMR). The structures of the major phospholipid (PL-2) from Thermus filiformis MOK14.7 and Meiothermus ruber WRG6.9 were identified as 2′-O-(1,2-diacyl-sn-glycero-3-phospho)-3′-O-(α-N-acetylglucosaminyl)-N-glyceroyl alkylamine (GlcNAc-PGAA) and 2′-O-(2-acylalkyldiol-1-O-phospho)-3′-O-(α-N-acetylglucosaminyl)-N-glyceroyl alkylamine (GlcNAc-diolPGAA). Interestingly, M. ruber contained only a diacyl form of GlcNAc-PGAA (87 %), while T. filiformis contained both GlcNAc-PGAA (59 %) and GlcNAc-diolPGAA (18 %). The structures of the minor phospholipid (PL-1) were established as 2′-O-(1,2-diacyl-sn-glycero-3-phospho)-3′-O-(α-glucosaminyl)-N-glyceroyl alkylamine (GlcN-PGAA, 13 %) in T. filiformis and 2′-O-(1,2-diacyl-sn-glycero-3-phospho)-3′-O-(α-galactosaminyl)-N-glyceroyl alkylamine (GalN-PGAA, 19 %) in M. ruber. This is the first reliable discovery of phosphatidylglyceroylalkylamines modified by glucosamine or galactosamine with a free amino group. No signs of diol-based phosphatidylglyceroylalkylamines were found in PL-1 phospholipids. Similar to PL-2, trace phospholipid (PL-0) from T. filiformis contained both unsubstituted diol-based phosphatidylglyceroylalkylamine (diolPGAA) and PGAA, while M. ruber contained only free PGAA. Unlike analysis using TLC, the diol form of phosphatidylglyceroylalkylamines is clearly resolved from the diacyl form via 31P-NMR. © 2014 AOCS. |
| URL | http://www.scopus.com/inward/record.url?eid=2-s2.0-84910602098&partnerID=40&md5=115f4cb6420b332e6998971093451303 |
| DOI | 10.1007/s11745-014-3946-z |
