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TitleHPLC and GC-MS identification of the major organic constituents in New Zealand propolis
Publication TypeJournal Article
Year of Publication1996
AuthorsMarkham, K.R., Mitchell K.A., Wilkins A.L., Daldy J.A., and Lu Y.
Pagination205 - 211
Date Published1996
ISSN00319422 (ISSN)
AbstractThe major organic constituents of New Zealand sourced propolis have been identified and quantified in tincture solutions by a combination of HPLC and GC-mass spectrometry (MS). HPLC was the preferred method for the analysis of flavonoids because of their very low response factors in GC-MS. Flavonoid levels ranged from 30-40 mg ml-1. A distinctive characteristic of the flavonoids in New Zealand propolis is the unusually high proportion (ca 70%) of dihydroflavonoids, e.g. pinocembrin, pinobanksin and pinobanksin 3-acetate. Non-flavonoid components analysed by GC-MS comprised a range of aromatic compounds (3-7.5 mg ml-1), together with low levels (0.25-0.78 mg ml-1) of fatty acids. The former comprised mainly cinnamic acids and their esters, but also included the rare 5-phenyl-trans-trans-2,4-pentadienoic acid and the new natural product, 5-phenyl-trans-3-pentenoic acid. Both were synthesized to confirm their identity. Copyright © 1996 Elsevier Science Ltd. All rights reserved.

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