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TitleEfficient Separation and Analysis of Triacylglycerols: Quantitation of β-Palmitate (OPO) in Oils and Infant Formulas
Publication TypeJournal Article
Year of Publication2015
AuthorsVyssotski, M., Bloor S.J., Lagutin K., Wong H., and Williams D.B.G.
JournalJournal of Agricultural and Food Chemistry
Pagination5985 - 5992
Date Published2015
ISSN00218561 (ISSN)
KeywordsELSD, Fatty Acids, Glycerol, high performance liquid chromatography, HPLC, Infant Formula, Isomers, Light scattering, Regiospecific analysis, Separation, Triacylglycerols
AbstractA high-efficiency, convenient, and reliable method for the separation of structurally similar triacylglycerols is detailed and applied in the quantitative analysis of 1,3-dioleoyl-2-palmitoylglycerol (OPO) in infant formulas and OPO oils. OPO is an important lipid component in humanized infant formula. A fast preparative isolation of an OPO-containing fraction from the crude complex mixture, by nonaqueous reversed phase HPLC, followed by Ag+-HPLC with detection at 205 nm allowed fine separation and detection of the desired fraction. OPO was quantitated independently of its regioisomer 1,2-dioleoyl-3-palmitoylglycerol (OOP) and isomers of stearoyl-linoleoyl-palmitoyl glycerol that might be present in infant formulas. For samples with low OPO content, an evaporative light-scattering detector (ELSD) was more preferable than UV detection, with a calculated LOD of 0.1 μg of OPO injected and LOQ of 0.3 μg. The method, which showed high reproducibility (RSD < 5%), was suitable for both high OPO content oils and low OPO products such as unenriched infant formula. A number of possible interference issues were considered and dealt with. © 2015 American Chemical Society.

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