Title | Efficient Separation and Analysis of Triacylglycerols: Quantitation of β-Palmitate (OPO) in Oils and Infant Formulas |
Publication Type | Journal Article |
Year of Publication | 2015 |
Authors | Vyssotski, M., Bloor S.J., Lagutin K., Wong H., and Williams D.B.G. |
Journal | Journal of Agricultural and Food Chemistry |
Volume | 63 |
Issue | 26 |
Pagination | 5985 - 5992 |
Date Published | 2015 |
ISSN | 00218561 (ISSN) |
Keywords | ELSD, Fatty Acids, Glycerol, high performance liquid chromatography, HPLC, Infant Formula, Isomers, Light scattering, Regiospecific analysis, Separation, Triacylglycerols |
Abstract | A high-efficiency, convenient, and reliable method for the separation of structurally similar triacylglycerols is detailed and applied in the quantitative analysis of 1,3-dioleoyl-2-palmitoylglycerol (OPO) in infant formulas and OPO oils. OPO is an important lipid component in humanized infant formula. A fast preparative isolation of an OPO-containing fraction from the crude complex mixture, by nonaqueous reversed phase HPLC, followed by Ag+-HPLC with detection at 205 nm allowed fine separation and detection of the desired fraction. OPO was quantitated independently of its regioisomer 1,2-dioleoyl-3-palmitoylglycerol (OOP) and isomers of stearoyl-linoleoyl-palmitoyl glycerol that might be present in infant formulas. For samples with low OPO content, an evaporative light-scattering detector (ELSD) was more preferable than UV detection, with a calculated LOD of 0.1 μg of OPO injected and LOQ of 0.3 μg. The method, which showed high reproducibility (RSD < 5%), was suitable for both high OPO content oils and low OPO products such as unenriched infant formula. A number of possible interference issues were considered and dealt with. © 2015 American Chemical Society. |
URL | http://www.scopus.com/inward/record.url?eid=2-s2.0-84936818693&partnerID=40&md5=5d141df7035b7f4256bb44a80dc60a4b |
DOI | 10.1021/acs.jafc.5b01835 |