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TitleEffect of storage regime on the stability of DNA used as a calibration standard for real-time polymerase chain reaction
Publication TypeJournal Article
Year of Publication2009
AuthorsPodivinsky, E., Love J.L., Colff L.v.d., and Samuel L.
JournalAnalytical Biochemistry
Volume394
Issue1
Pagination132 - 134
Date Published2009
ISSN00032697 (ISSN)
Keywordsaqueous solution, article, bacterial DNA, Calibration, Campylobacter jejuni, controlled study, cryopreservation, DNA, DNA sequence, DNA, Bacterial, Drug Storage, freeze drying, Gastrointestinal Diseases, molecular stability, nonhuman, Polymerase Chain Reaction, priority journal, Public Health, quantitative analysis, real time polymerase chain reaction, Specimen Handling, storage temperature, Time Factors
AbstractThis article looks at storage factors influencing the stability of potential DNA calibration standards for use in quantitative polymerase chain reaction (PCR). Target sequences from the bacteria Campylobacter jejuni were cloned into a plasmid vector. Samples of these potential calibration standards were stored at +4, -20, and -80 °C as aqueous and lyophilized samples and were prepared as both single-use aliquots and multiple-use preparations. Results showed that the samples stored as single-use aqueous solutions at +4 °C and lyophilized samples stored at +4 and -20 °C were the most stable. Samples stored as frozen aqueous solutions at -20 °C were the least stable. © 2009 Elsevier Inc. All rights reserved.
URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-69249216418&partnerID=40&md5=3e20d43cf962b9bfe78986d84eef8245
DOI10.1016/j.ab.2009.06.024

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