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TitleEcophysiological diversity of a novel member of the genus Alteromonas, and description of Alteromonas mediterranea sp. nov.
Publication TypeJournal Article
Year of Publication2015
AuthorsIvanova, E.P., López-Pérez M., Zabalos M., Nguyen S.H., Webb H.K., Ryan J., Lagutin K., Vyssotski M., Crawford R.J., and Rodriguez-Valera F.
JournalAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Pagination119 - 132
Date Published2015
ISSN00036072 (ISSN)
KeywordsAlteromonas, Atlantic Ocean, Bacterial Typing Techniques, chemistry, Classification, Cluster analysis, DNA, Bacterial, DNA, Ribosomal, genetics, Genome, Bacterial, Locomotion, Mediterranean Sea, microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, phylogeny, physiology, RNA, Ribosomal, 16S, Seawater, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid, Sodium chloride
AbstractNine non-pigmented, motile, Gram-negative bacteria originally designated as Alteromonas macleodii deep-sea ecotypes, were isolated from seawater samples collected from four separate locations; two deep-sea sites in the Mediterranean Sea and surface water of the Aegean Sea and English Channel. The six strains studied in vitro were found to tolerate up to 20 % NaCl. The DNA–DNA relatedness between the deep-sea ecotype strains was found to be between 75 and 89 %, whilst relatedness with the validly named Alteromonas species was found to be between 31 and 69 %. The average nucleotide identity (ANI) amongst the deep-sea ecotype strains was found to be 98–100 %; the in silico genome-to-genome distance (GGD), 85–100 %; the average amino acid identity (AAI) of all conserved protein-coding genes, 95–100 %; and the strains possessed 30–32 of the Karlin’s genomic signature dissimilarity. The ANI between the deep-sea ecotype strains and A. macleodii ATCC 27126T and Alteromonas australica H 17T was found to be 80.6 and 74.6 %, respectively. A significant correlation was observed between the phenotypic data obtained in vitro and data retrieved in silico from whole genome sequences. The results of a phylogenetic study that incorporated a 16S rRNA gene sequence analysis, multilocus phylogenetic analysis (MLPA) and genomic analysis, together with the physiological, biochemical and chemotaxonomic data, clearly indicated that the group of deep-sea ecotype strains represents a distinct species within the genus Alteromonas. Based on these data, a new species, Alteromonas mediterranea, is proposed. The type strain is DET ( = CIP 110805T = LMG 28347T = DSM 17117T). © 2014, Springer International Publishing Switzerland.

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