| Title | Discovery of lipids from B. longum subsp. infantis using whole cell MALDI analysis |
| Publication Type | Journal Article |
| Year of Publication | 2014 |
| Authors | Timmer, M.S.M., Sauvageau J., Foster A.J., Ryan J., Lagutin K., Shaw O., Harper J.L., Sims I.M., and Stocker B.L. |
| Journal | Journal of Organic Chemistry |
| Volume | 79 |
| Issue | 16 |
| Pagination | 7332 - 7341 |
| Date Published | 2014 |
| ISSN | 00223263 (ISSN) |
| Keywords | acetal glycolipid, article, Bacteria, Bacterial Adhesion, bacterial cell, bacterium adherence, Bifidobacteria, Bifidobacterium, Bifidobacterium longum, Bifidobacterium longum infantis, Bioactivity, carbon nuclear magnetic resonance, Cell membranes, chemistry, fatty acid, Glycerol, glycolipid, Glycolipids, heteronuclear multiple bond correlation, heteronuclear single quantum coherence, human, Humans, immune response, Immune system, immunology, Innate immune response, innate immunity, Intestinal tract, intestine, Intestines, lipid, lipid analysis, lipid composition, Lipids, Mass spectrometry, metabolism, Microbial communities, microbiology, nonhuman, phosphatidic acid, plasmalogen, plasmenyl cyclophosphatidic acid, proton nuclear magnetic resonance, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Structural characterization, Structural elucidation, sulfalene, unclassified drug, whole cell |
| Abstract | Bifidobacteria are dominant members of the microbial community in the intestinal tract of infants, and studies have shown that glycolipids extracted from the cell surface of these bacteria elicit beneficial immune responses. Accordingly, the identification and structural characterization of glycolipids from the cell wall of bifidobacteria is the first step in correlating glycolipid structure with biological activity. Using whole cell MALDI as a screening tool, we herein present for the first time the identification and structural elucidation of the major polar lipids from Bifidobacterium longum subs. infantis. The lipids identified include an unprecedented plasmenyl cyclophosphatidic acid and a mixed acetal glycolipid, with the latter subsequently being isolated and found to suppress the innate immune response. © 2014 American Chemical Society. |
| URL | http://www.scopus.com/inward/record.url?eid=2-s2.0-84906085550&partnerID=40&md5=3d7472e241d4ca1a05f99133b34134d4 |
| DOI | 10.1021/jo501016c |
