| Title | Crystallization and preliminary X-ray diffraction analysis of dihydrodipicolinate synthase 2 from Arabidopsis thaliana |
| Publication Type | Journal Article |
| Year of Publication | 2011 |
| Authors | Griffin, M.D.W., Billakanti J.M., Gerrard J.A., Dobson R.C.J., and Pearce F.G. |
| Journal | Acta Crystallographica Section F: Structural Biology and Crystallization Communications |
| Volume | 67 |
| Issue | 11 |
| Pagination | 1386 - 1390 |
| Date Published | 2011 |
| ISSN | 17443091 (ISSN) |
| Keywords | Animalia, Arabidopsis, Arabidopsis thaliana, article, chemistry, Crystallization, Crystallography, X-Ray, dihydrodipicolinate synthase, enzymology, Hydro-Lyases, hydrolyase, X ray crystallography |
| Abstract | Dihydrodipicolinate synthase (DHDPS; EC 4.2.1.52) catalyzes the first committed step of the lysine-biosynthetic pathway in plants and bacteria. Since (S)-lysine biosynthesis does not occur in animals, DHDPS is an attractive target for rational antibiotic and herbicide design. Here, the cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DHDPS2 from Arabidopsis thaliana are reported. Diffraction-quality protein crystals belonged to space group P21212. © 2011 International Union of Crystallography. All rights reserved. |
| URL | http://www.scopus.com/inward/record.url?eid=2-s2.0-80955135722&partnerID=40&md5=e6f994685fbf78ad298e4657ead4fcb1 |
| DOI | 10.1107/S1744309111033276 |
