Callaghan Innovation Research Papers

Back to Research Papers

TitleChthonomonas calidirosea gen. nov., sp. nov., an aerobic, pigmented, thermophilic micro-organism of a novel bacterial class, Chthonomonadetes classis nov., of the newly described phylum Armatimonadetes originally designated candidate division OP10
Publication TypeJournal Article
Year of Publication2011
AuthorsLee, K.C.-Y., Dunfield P.F., Morgan X.C., Crowe M.A., Houghton K.M., Vyssotski M., Ryan J.L.J., Lagutin K., McDonald I.R., and Stott M.B.
JournalInternational Journal of Systematic and Evolutionary Microbiology
Pagination2482 - 2490
Date Published2011
ISSN14665026 (ISSN)
KeywordsAerobiosis, Armatimonadetes, article, Bacteria, Bacteria (microorganisms), bacterial DNA, bacterial genetics, bacterial growth, bacterial membrane, Bacterial Physiological Phenomena, bacterial RNA, bacterial strain, bacterium isolation, Base Composition, Carbohydrate Metabolism, catalase, cellobiose, Cellulose, Chthonomonadetes, Chthonomonas calidirosea, Cluster analysis, concentration (parameters), controlled study, cotton, DNA base composition, DNA, Bacterial, DNA, Ribosomal, fatty acid, Fatty Acids, genomic DNA, glucose, glycogen, Gossypium, Gossypium hirsutum, Gram negative aerobic rods and cocci, Gram staining, growth rate, high temperature, Hydrogen-Ion Concentration, lactose, Locomotion, mannose, Molecular Sequence Data, Negibacteria, new zealand, nonhuman, Oxidoreductases, pH, phenotype, phylogeny, phylum, priority journal, quinone derivative, Quinones, RNA 16S, RNA sequence, RNA, Ribosomal, 16S, Sequence Analysis, DNA, Sodium chloride, starch, strain identification, taxonomic identification, Temperature, thermophilic bacterium, xylan
AbstractAn aerobic, saccharolytic, obligately thermophilic, motile, non-spore-forming bacterium, strain T49 T, was isolated from geothermally heated soil at Hell's Gate, Tikitere, New Zealand. On the basis of 16S rRNA gene sequence similarity, T49 T is the first representative of a new class in the newly described phylum Armatimonadetes, formerly known as candidate division OP10. Cells of strain T49 T stained Gram-negative and were catalase-positive and oxidase-negative. Cells possessed a highly corrugated outer membrane. The major fatty acids were 16: 0, i17: 0 and ai17: 0. The G+C content of the genomic DNA was 54.6 mol%. Strain T49T grew at 50-73 °C with an optimum temperature of 68 °C, and at pH 4.7-5.8 with an optimum growth pH of 5.3. A growth rate of 0.012 h -1 was observed under optimal temperature and pH conditions. The primary respiratory quinone was MK-8. Optimal growth was achieved in the absence of NaCl, although growth was observed at NaCl concentrations as high as 2% (w/v). Strain T49T was able to utilize mono- and disaccharides such as cellobiose, lactose, mannose and glucose, as well as branched or amorphous polysaccharides such as starch, CM-cellulose, xylan and glycogen, but not highly linear polysaccharides such as crystalline cellulose or cotton. On the basis of its phylogenetic position and phenotypic characteristics, we propose that strain T49 T represents a novel bacterial genus and species within the new class Chthonomonadetes classis nov. of the phylum Armatimonadetes. The type strain of Chthonomonas calidirosea gen. nov., sp. nov. is T49 T (=DSM 23976 T=ICMP 18418 T). © 2011 Crown.

Back to top