| Title | Calibration of UV-vis spectrophotometers for chemical analysis |
| Publication Type | Journal Article |
| Year of Publication | 2005 |
| Authors | Clare, J.F. |
| Journal | Accreditation and Quality Assurance |
| Volume | 10 |
| Issue | 6 |
| Pagination | 283 - 288 |
| Date Published | 2005 |
| ISSN | 09491775 (ISSN) |
| Keywords | Accuracy, analytic method, Calibration, Chemical analysis, Light absorption, reproducibility, review, sensitivity and specificity, ultraviolet spectrophotometry |
| Abstract | The spectrophotometer has become the workhorse for high-throughput routine testing for a wide variety of analytes in chemical and biochemical analysis laboratories. It is important for accuracy and reproducibility in measurement that this instrument is correctly set up and calibrated, a task which is facilitated by an understanding of the principles employed. This presentation reviews the principal issues involved: absorbance calibration, wavelength calibration, and checks for both heterochromatic and isochromatic stray light. Methods and materials for making these checks are compared and discussed. © Springer-Verlag 2005. |
| URL | http://www.scopus.com/inward/record.url?eid=2-s2.0-21744432999&partnerID=40&md5=d67abad9d5031f6538a4a99989ffd06c |
| DOI | 10.1007/s00769-005-0927-1 |
