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TitleCalibration of UV-vis spectrophotometers for chemical analysis
Publication TypeJournal Article
Year of Publication2005
AuthorsClare, J.F.
JournalAccreditation and Quality Assurance
Pagination283 - 288
Date Published2005
ISSN09491775 (ISSN)
KeywordsAccuracy, analytic method, Calibration, Chemical analysis, Light absorption, reproducibility, review, sensitivity and specificity, ultraviolet spectrophotometry
AbstractThe spectrophotometer has become the workhorse for high-throughput routine testing for a wide variety of analytes in chemical and biochemical analysis laboratories. It is important for accuracy and reproducibility in measurement that this instrument is correctly set up and calibrated, a task which is facilitated by an understanding of the principles employed. This presentation reviews the principal issues involved: absorbance calibration, wavelength calibration, and checks for both heterochromatic and isochromatic stray light. Methods and materials for making these checks are compared and discussed. © Springer-Verlag 2005.

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