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TitleBond-rupture immunosensors-A review
Publication TypeJournal Article
Year of Publication2008
AuthorsHirst, E.R., Yuan Y.J., Xu W.L., and Bronlund J.E.
JournalBiosensors and Bioelectronics
Pagination1759 - 1768
Date Published2008
ISSN09565663 (ISSN)
KeywordsAgricultural monitoring, agriculture, Amplitude modulation, antigen antibody complex, Antigen-antibody reactions, Bacteria, bacterium detection, Biosensing Techniques, biosensor, Bond rupture, Cost effectiveness, drug research, Electrochemistry, electronics, elementary particle, environmental monitoring, Equipment Design, food analysis, force, immune response, Immunoassay, immunosensor, Immunosensors, laboratory test, molecular interaction, noise, oscillation, Piezoelectricity, Point of care testing, qualitative analysis, quantitative analysis, Quartz crystal microbalances, receptor, review, silicon dioxide, Technology Assessment, Biomedical, theoretical model, Transducers, virus detection
AbstractIt has long been the goal of researchers to develop fast and reliable point-of-care alternatives to existing lab-based tests. A viable point-of-care biosensor is fast, reliable, simple, cost-effective, and detects low concentrations of the target analyte. The target of biosensors is biological such as bacteria or virus and as such, the antibody-antigen bond derived from the real immune response is used. Biosensor applications include lab-based tests for the purposes of diagnostics, drug discovery, and research. Additional applications include environmental, food, and agricultural monitoring. The main merits of the bond-rupture method are quick, simple, and capable of discriminating between specific and non-specific interactions. The separation of specific and non-specific bonds is important for working in real-life complex serums such as blood. The bond-rupture technique can provide both qualitative results, the detection of a target, and quantitative results, the concentration of target. Bond-rupture achieves this by a label-free method requiring no pre-processing of the analyte. A piezoelectric transducer such as the quartz crystal microbalance (QCM) shakes the bound particles free from the surface. Other transducers such as Surface Acoustic Wave (SAW) are also considered. The rupture of the bonds is detected as electronic noise. This review article links diverse research areas to build a picture of a field still in development. © 2008 Elsevier B.V. All rights reserved.

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