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TitleArenibacter gen. nov., new genus of the family Flavobacteriaceae and description of a new species, Arenibacter latericius sp. nov.
Publication TypeJournal Article
Year of Publication2001
AuthorsIvanova, E.P., Nedashkovskaya O.I., Chun J., Lysenko A.M., Frolova G.M., Svetashev V.I., Vysotskii M.V., Mikhailov V.V., Huq A., and Colwell R.R.
JournalInternational Journal of Systematic and Evolutionary Microbiology
Pagination1987 - 1995
Date Published2001
ISSN14665026 (ISSN)
Keywordsaerobic bacterium, alga, algae, Algae, Brown, Animals, Apostichopus, Apostichopus japonicus, Arenibacter, Arenibacter latericius, article, Bacteria (microorganisms), bacterial metabolism, bacterium isolation, Base Composition, branched chain amino acid, China, Chorda filum, controlled study, culture medium, cytosine, DNA base composition, DNA sequence, DNA, Ribosomal, extract, fatty acid, Fatty Acids, Flavobacteriaceae, Flavobacterium, Geologic Sediments, Gram negative bacterium, Gram-Negative Aerobic Rods and Cocci, guanine, Holothuria, Indian Ocean, Japan, lipid, lipid analysis, methodology, Molecular Sequence Data, morphology, Negibacteria, nonhuman, nucleotide sequence, Pacific Ocean, Phaeophyceae, phenotype, phosphatidylethanolamine, phylogeny, Pigments, Biological, priority journal, Prokaryota, ribosome DNA, RNA, Ribosomal, 16S, sand, saturated fatty acid, sea, Sea Cucumbers, sea water, Seawater, sediment, sequence homology, taxonomy, unsaturated fatty acid
AbstractFive dark-orange-pigmented, Gram-negative, rod-shaped, non-motile, aerobic bacterial strains were isolated from sandy sediment samples collected in the South China Sea in the Indian Ocean, from a holothurian, Apostichopus japonicus, in the Sea of Japan and from a brown alga, Chorda filum, from the Sea of Okhotsk in the Pacific Ocean. Phenotypic data were collected, demonstrating that the bacteria are chemo-organotrophic and require seawater-based media for growth. Polar lipids were analysed and 27% of the total extract comprised phosphatidylethanolamine as the major component. The predominant cellular fatty acids were branched-chain saturated and unsaturated [i-C15:0, i-C15:1, a-C15:0, C15:0, C16:1(n-7)]. The DNA base composition was 37.5-38.2 mol% G+C. The level of DNA homology of the five isolates was 83-94%, indicating that these isolates belong to the same species. A 16S rDNA sequence of the type strain KMM 426T was determined and phylogenetic analysis, based on neighbour-joining and Fitch-Margoliash methods, revealed that the type strain formed a distinct phyletic line in a clade corresponding to the family Flavobacteriaceae and represented a new genus. From the results of this polyphasic taxonomic analysis, it is proposed that the bacterial strains be classified in a new genus, Arenibacter gen. nov., and species, Arenibacter latericius sp. nov. The type strain is KMM 426T (VKM B 2137DT = LMG 19694T = CIP 106861T).

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